Open Water Swimming in Elite Triathletes: Physiological and Biomechanical Determinants.

This study aimed (i) to analyze the 1500 m open water swimming performance, (ii) to examine the associations between physiological and biomechanical variables with swimming performance, and (iii) to determine which variables can predict swimming performance in triathletes. Fourteen elite triathletes (23.4±3.8 y) performed a 1500 m test in open water swimming conditions. Swimming performance was assessed using World Aquatics Points Scoring, and data were obtained from the 1500 m open water swimming test. Heart rate, end-exercise oxygen uptake (EE˙VO2) and blood lactate concentrations were measured. The initial 250 m of the 1500 m swimming test presented the highest values of biomechanical variables in males (i. e. swimming speed, stroke rate (SR), length (SL), index (SI)). A decrease in SL was observed in the last 250 m in both sexes. Positive association were found between EE˙VO2 (r=0.513; p=0.030), swimming speed (r=0.873; p<0.001) and SI (r=0.704; p=0.002) with swimming performance. In contrast, time constant of the oxygen uptake (r=-0.500; p=0.034) and buoy-turn times (r=-0.525; p=0.027) were negatively associated with performance. SI was the main predictor (R 2=0.495) of open water swimming performance in triathletes. In conclusion, triathletes and coaches must conduct open water training sessions to maximize SI (i. e. swimming efficiency).

Power Profile during Cycling in World Triathlon Series and Olympic Games.

This study aimed to analyze the power profile (PP) during the cycling segment of international-level triathletes in the World Triathlon Series (WTS) and Olympics and to evaluate the influence of circuit type, race distance (Sprint or Olympic distance) and race dynamics on the development of the cycling leg and the final race position. Four male triathletes participated in the study. Twenty races were analyzed using geolocation technology and power-meter data to analyze PP, race dynamics, and course characteristics. Before the races, incremental tests of volitional exhaustion with gas analysis were performed to determine power intensity zones. Nonparametric Mann-Whitney U tests and correlation analyses were conducted to identify differences and relationships between various variables. A correlation between the time spent above maximal aerobic power (MAP) and dangerous curves per kilometer (r = 0.46; p < 0.05) and bike split result (BSR) (r = -0.50; p < 0.05) was observed. Also, moderate correlation was found between BSR and the final race position (r = 0.46; p < 0.01). No differences were found between sprint and Olympic distance races in any variable. Power output variability, influenced by technical circuit segments, remains the main characteristic in international short-distance races. The results of the present study suggest that the triathletes who are better adapted to intermittent high intensity efforts perform better cycling legs at international high-level races.

Training Characteristics, Performance, and Body Composition of Three U23 Elite Female Triathletes throughout a Season.

(1) Background: There is a lack of data on the long-term training characteristics and performance markers of elite young female endurance athletes. The aim of this study was to present the training load (ECOs), as well as the evolution of the anthropometric values and performance of three elite U23 female triathletes over a season. (2) Methods: General training data and performance data relating to the swimming, cycling, and running legs of the 2021 season were described. The training intensity distribution (TID) was presented using the triphasic model, while the training load was based on the ECO model. An anthropometric analysis was also conducted in accordance with the ISAK standards. (3) Results: Triathletes increased their VO2max in cycling (6.9-10%) and running (7.1-9.1%), as well as their power and speed associated with the VO2max (7.7-8.6% in cycling and 5.1-5.3% in running) and their swimming speed associated with the lactate thresholds (2.6-4.0% in LT2 and 1.2-2.5% in LT1). The triathletes completed more than 10 h of weekly average training time, with peak weeks exceeding 15 h. The average TID of the three triathletes was 82% in phase 1, 6% in phase 2, and 12% in phase 3. A decrease in the sum of skinfolds and fat mass percentage was observed during the season in the three triathletes, although the last measurement revealed a stagnation or slight rise in these parameters. (4) Conclusions: The triathletes performed a combination of two training periodization models (traditional and block periodization) with a polarized TID in most of the weeks of the season. Improvements in performance and physiological parameters were observed after the general preparatory period as well as a positive body composition evolution throughout the season, except at the end, where the last measurement revealed stagnation or a slight decline. This study can be useful as a general guide for endurance coaches to organize a training season with female U23 triathletes.

Does Pool Performance of Elite Triathletes Predict Open-Water Performance?

The capacity of laboratory tests to predict competition performance has been broadly researched across several endurance sports. The aim of the present study was to analyse how pool swimming performance can predict the result of the swimming segment in triathlon competitions and compare predictability differences based on competition level and distance. Eighteen male triathletes participated in the study. Three were ranked world-class, ten elite/international level, and five highly trained/national level. A total of sixty-one graded multi-stage swimming tests were conducted. Blood lactate was measured to calculate the following hypothetical predictor variables: speed at lactate threshold 1 (LT1), speed at lactate threshold 2 (LT2), and speed in the last repetition of the test (SL200). The following data were collected for a total of 75 races: time in the swimming leg (TSL); position after the swimming leg (PSL); time difference with the first triathlete after the swimming leg (DFT); and final race position. The race levels were divided according to participant levels as follows: world series (WS) (n = 22); World Cup (WC) (n = 22); Continental Cup (CC) (n = 19); national championship (N) (n = 5); and local race (L) (n = 5). Based on distance, they were divided into Olympic distance (OD) (n = 37) and sprint distance (SD) (n = 38). A moderate to strong positive association was found between LT1, LT2, SL200 and PSL and TSl at all race levels except for the SD CC, SD WC, and OD CC races, where no or weak-to-moderate correlations were found. The present study demonstrated that performance measured in a graded multi-stage pool lactate test can predict performance in a triathlon swimming segment. This finding is highly useful for coaches as it can help them to obtain a reliable measure of the triathlete's specific capabilities in the swimming leg.

Determinants of 1500-m Front-Crawl Swimming Performance in Triathletes: Influence of Physiological and Biomechanical Variables.

PURPOSE: To analyze the associations between physiological and biomechanical variables with the FINA (International Swimming Federation) points (ie, swimming performance) obtained in 1500-m front-crawl swimming to determine whether these variables can be used to explain triathletes' FINA points. METHODS: Fourteen world-class, international and national triathletes (10 male: 23.24 [3.70] y and 4 female: 23.36 [3.76] y) performed a 1500-m front-crawl swimming test in a short-course pool. Heart rate (HR), oxygen uptake (V˙O2), and blood lactate concentrations were obtained before and after the test. HR was also measured during the effort. Highest V˙O2 value (V˙O2peak) was estimated by extrapolation. Clean swimming speed, turn performance, stroke rate, stroke length, and stroke index (SI) were obtained by video analysis. RESULTS: Average 1500-m performance times were 1088 (45) seconds and 1144 (31) seconds for males and females, respectively. HR after the effort, V˙O2peak, aerobic contributions, total energy expenditure, energy cost, and turn performance presented moderate negative associations with swimming performance (r ≈ .5). In contrast, respiratory exchange ratio, anaerobic alactic contribution, clean swimming speed, stroke length, and SI were positively related, with clean swimming speed and SI having a strong large association (r ≈ .7). A multiple stepwise regression model determined that 71% of the variance in FINA points was explained by SI and total energy expenditure, being predictors in 1500-m front-crawl swimming. CONCLUSIONS: Swimming performance in triathletes was determined by the athletes' energy demands and biomechanical variables. Thus, coaches should develop specific technique skills to improve triathletes' swimming efficiency.

Training characteristics and performance of two male elite short-distance triathletes: From junior to "world-class".

OBJECTIVE: The sports-science literature lacks data on training and performance characteristics of international elite athletes over multiple seasons. The present case study provided general training characteristics and performance data of two male short-distance triathletes in the Junior, U23, and international Elite categories. METHODS: General training and performance data of two male elite triathletes were described in swimming, cycling, and running segments from the 2015 to 2022 season. The training load was presented using the ECO model while the training intensity distribution (TID) was a triphasic model. RESULTS: Both triathletes increased their performance throughout the seasons. Triathlete A increased his VO2max in cycling by 20.6%, in running by 16.7%. His power at VO2max and his speed at VO2max by 18.9% and 11.0%, respectively. Triathlete B improved his VO2max by 17.8% in cycling, by 16.1% in running and his power at VO2max by 24%, and his speed at VO2max by 14.3%. The triathletes trained on average 14-17 h a week. The TID model was polarized. CONCLUSIONS: To achieve the top international level, it is necessary to consider the following measures: training load progression; improvements in physiological variables; and participation in international events starting from youth categories.

Road to Tokyo 2020 Olympic Games: Training Characteristics of a World Class Male Triathlete.

There is a growing interest in the scientific literature for reporting top-class endurance athletes training programs. This case study reports on the training program of a world-class male triathlete preparing to compete in the Tokyo 2020 Olympic Games. A macrocycle of 43 weeks is presented. The triathlete performed 14.74 ± 3.01 h of weekly endurance training volume. Training intensity distribution (TID) was 81.93% ± 6.74%/7.16% ± 2.03%/10.91% ± 6.90% for zones 1 (low intensity, VT2) respectively. Pyramidal TID model is observed during the initial stages of the periodization and Polarized TID model is observed at the end of the macrocycle. The triathlete's peak ⩒O2 was increased by 20% on cycling and by 14% on running. Peak power was increased by 3.13% on cycling test and peak speed by 9.71% on running test. Finally, the triathlete placed 12th in Olympic distance and 10th in Mixed Relay in Tokyo 2020 Olympic games.

Using Musical Feedback Increases Stride Frequency in Recreational Runners.

The number of participants in popular races has increased in recent years, with most of them being amateurs. In addition, it has been observed that there is a high percentage of injuries among them, and some of these injuries may be related to a low stride frequency. The aim of this research was to check if a continuous running training program with a musical base improves the stride frequency of popular runners. For this purpose, the effect of a 6 week continuous running training program with the help of a musical track with a constant rhythm that was 10% higher than the preferred stride frequency of the subjects was analyzed and compared to a control group that performed the continuous running training without sound stimuli. Significant increases were found in the evolution of stride frequency in the experimental group between the pre- and post-test (p = 0.002). No significant changes were observed in the stride frequency of the control group. These results show that training with music feedback helps to improve stride frequency in recreational runners. Future research should study the evolution of the improvement obtained in time as it is unknown if the increase in stride rate has been integrated in the runner's technique, making the improvement obtained permanent. Future research is needed to confirm these results by enlarging the sample and carrying out an exhaustive biomechanical study.

Muscle Activation in Middle-Distance Athletes with Compression Stockings.

The aim of this study was to evaluate changes in electromyographic activity with the use of gradual compression stockings (GCSs) on middle-distance endurance athletes' performance, based on surface electromyography measurement techniques. Sixteen well-trained athletes were recruited (mean ± SD: age 33.4 ± 6.3 years, VO2max 63.7 ± 6.3 mL·kg-1·min-1, maximal aerobic speed 19.7 ± 1.5 km·h). The athletes were divided into two groups and were assigned in a randomized order to their respective groups according to their experience with the use of GCSs. Initially, a maximum oxygen consumption (VO2max) test was performed to standardize the athletes' running speeds for subsequent tests. Afterward, electromyographic activity, metabolic, and performance variables for each group were measured with surface electromyography. In addition, blood lactate concentration was measured, both with and without GCSs, during 10 min at 3% above VT2 (second ventilatory threshold), all of which were performed on the track. Next, surface electromyography activity was measured during a 1 km run at maximum speed. No significant changes were found in electromyography activity, metabolic and performance variables with GCSs use (p > 0.164) in any of the variables measured. Overall, there were no performance benefits when using compression garments against a control condition.

Polarized and Pyramidal Training Intensity Distribution: Relationship with a Half-Ironman Distance Triathlon Competition.

The purpose of the present study was to relate the training intensity distribution with performance in a Half-Ironman distance triathlon competition. A total of 18 recreational-level triathletes were divided into two training groups according to their training intensity distribution: Polarized (POL) and Pyramidal (PYR). Prior to the specific training period of the study, subjects performed a ramp-protocol test, running and cycling to determine ventilatory thresholds (VT) through gas-exchange analysis. For swimming, subjects performed an 800-metre test to establish their training zones. Training was quantified based on the cumulative time spent in 3 intensity zones: zone 1 (low intensity, VT2). POL competed 84.5%/4.2%/11.3% and PYR 77.9%/18.8%/3.3% of total training time for zones 1,2 and 3 respectively. The goal of the training period was a half Ironman distance triathlon. Training time in zone 2 inversely correlated with swimming and cycling race time in POL and with running and total race time in PYR. Power at VT2 on bike and speed at VT2 as well as maximum aerobic power and speed in the physiological post test inversely correlated with bike and run segment respectively and with total race time. These results suggest that training time in zone 2 was related with better performance on a Half-Ironman race in amateur triathletes. Future experimental research is needed to clarify the importance of training intensity distribution regarding performance.

Changes in Triathletes' Performance and Body Composition During a Specific Training Period for a Half-Ironman Race.

The number of recreational athletes completing a Half-Ironman triathlon has increased exponentially in recent years. However, there is a lack of research on how to train for this kind of an event. The purpose of this study was thus to analyse triathletes' changes in performance and body composition following a triathlon-specific training period. Fourteen male amateur triathletes completed a 7-week period of general training and a 13-week period of specific training for a Half-Ironman triathlon. Anthropometric measures and performance tests were carried out to assess the effects of the specific training program. Results showed that the pre-test value of VO2max for cycling was inversely correlated not only with the percentage of change in cycling performance, but also with the percentage change in several variables of running performance. In swimming, inverse correlations were observed between the time of the first 800 m test and the time percentage change for this test, but not with the percentage change in the performance of other segments of the race. Moreover, the somatotype component of endomorphy and the fat mass percentage of the first anthropometry were highly correlated with the percentage change in VO2max in the run segment. These results highlight the importance of providing individualised training, considering that the same training program had a different impact on recreational triathletes belonging to the same group. Amateur athletes with higher initial performance levels probably need a greater amount of training to achieve improved adaptation.

Effect of Different Feedback Modalities on Swimming Pace: Which Feedback Modality is Most Effective?

To compare the effect of three different feedback modalities on swimming pace, sixteen male swimmers and triathletes participated in this study. Each participant swam 3 x 400 m, one for each feedback modality, swimming front crawl at 80% of their individual swimming critical speed. Three feedback modalities were examined: self-pacing, real time visual feedback and real time voice feedback. The swimmers adopted a fast start in all feedback modalities. In the real time voice feedback modality, the data recorded during the second lap (200 m) showed a significant improvement of their swimming pace approaching the swimming pace intended (-1.47 s, p < .01, medium effect size 0.79). A significant improvement toward the swimming pace intended was also noticed at the third split time (300 m) (0.05 s, p < .01, large effect size 0.81) and at the fourth split time (400 m) (0.46 s, p < .01, medium effect size 0.76). In self-pacing, the swimmers were not able to swim in line with the swimming pace intended. In real time visual feedback modality, the swimmers did not show a significant improvement approaching the swimming pace intended. The results revealed that communication with the swimmers using the real time voice feedback induced a significant improvement in their swimming pace and could help the athletes to swim with accurate and consistent pace.

Aronia-citrus juice (polyphenol-rich juice) intake and elite triathlon training: a lipidomic approach using representative oxylipins in urine.

In the present study, we examined whether particular urinary oxylipins (isoprostanes (IsoPs), leukotrienes (LTs), prostaglandins (PGs), and thromboxanes (TXs)) in 16 elite triathletes could alter during 145 days of training. Within this time span, 45 days were dedicated to examining the effects of the intake of a beverage rich in polyphenols (one serving: 200 mL per day) supplemented in their diet. The beverage was a mixture of citrus juice (95%) and Aronia melanocarpa juice (5%) (ACJ). Fifty-two oxylipins were analyzed in the urine. The quantification was carried out using solid-phase extraction, liquid chromatography coupled with triple quadrupole mass spectrometry. The physical activity decreased the excretion of some PG, IsoP, TX, and LT metabolites from arachidonic acid, γ-dihomo-linolenic acid, and eicosapentaenoic acid. The ACJ also reduced the excretion of 2,3-dinor-11ß-PGF2α and 11-dehydro-TXB2, although the levels of other metabolites increased after juice supplementation (PGE2, 15-keto-15-F2t-IsoP, 20-OH-PGE2, LTE4, and 15-epi-15-E2t-IsoP), compared to the placebo. The metabolites that increased in abundance have been related to vascular homeostasis and smooth muscle function, suggesting a positive effect on the cardiovascular system. In conclusion, exercise influences mainly the decrease in oxidative stress and the inflammation status in elite triathletes, while ACJ supplementation has a potential benefit regarding the cardiovascular system that is connected in a synergistic manner with elite physical activity.

Is Marathon Training Harder than the Ironman Training? An ECO-method Comparison.

Purpose: To compare the absolute and relative training load of the Marathon (42k) and the Ironman (IM) training in recreational trained athletes. Methods: Fifteen Marathoners and Fifteen Triathletes participated in the study. Their performance level was the same relative to the sex's absolute winner at the race. No differences were presented neither in age, nor in body weight, height, BMI, running VO2max max, or endurance training experience (p > 0.05). They all trained systematically for their respective event (IM or 42k). Daily training load was recorded in a training log, and the last 16 weeks were compared. Before this, gas exchange and lactate metabolic tests were conducted in order to set individual training zones. The Objective Load Scale (ECOs) training load quantification method was applied. Differences between IM and 42k athletes' outcomes were assessed using Student's test and significance level was set at p < 0.05. Results: As expected, Competition Time was significantly different (IM 11 h 45 min ± 1 h 54 min vs. 42k 3 h 6 min ± 28 min, p < 0.001). Similarly, Training Weekly Avg Time (IM 12.9 h ± 2.6 vs. 42k 5.2 ± 0.9), and Average Weekly ECOs (IM 834 ± 171 vs. 42k 526 ± 118) were significantly higher in IM (p < 0.001). However, the Ratio between Training Load and Training Time was superior for 42k runners when comparing ECOs (IM 65.8 ± 11.8 vs. 42k 99.3 ± 6.8) (p < 0.001). Finally, all ratios between training time or load vs. Competition Time were superior for 42k (p < 0.001) (Training Time/Race Time: IM 1.1 ± 0.3 vs. 42k 1.7 ± 0.5), (ECOs Training Load/Race Time: IM 1.2 ± 0.3 vs. 42k 2.9 ± 1.0). Conclusions: In spite of IM athletes' superior training time and total or weekly training load, when comparing the ratios between training load and training time, and training time or training load vs. competition time, the preparation of a 42k showed to be harder.

Lipidomic approach in young adult triathletes: effect of supplementation with a polyphenols-rich juice on neuroprostane and F2-dihomo-isoprostane markers.

The aim of the this study was to determine the effect of a polyphenols-rich juice (aronia-citrus juice, ACJ) on F4-neuroprostanes and F2-dihomo-isoprostanes-markers of oxidative stress associated with the central nervous system (CNS)-in 16 elite triathletes under a controlled diet for triathlon training (145 days). In the triathletes, a decrease of the lipid peroxidation markers after ACJ intake, associated with neuronal membrane degradation (10-epi-10-F4t-neuroprostane and 10-F4t-neuroprostane), was observed when compared with placebo stage values. Regarding the F2-dihomo-isoprostanes, a significant decrease of the neuromotor system damage biomarkers (17-F2t-dihomo-isoprostane) with an increase of training load during the study was observed, although the decrease of the load training at the last stage showed a significant increase of the values of ent-7-(RS)-7-F2t-dihomo-IsoP, suggesting a possible role in adaptation post-training. On the other hand, the changes in the excretion of 17-epi-17-F2t-dihomo-IsoP provided a positive connection between physical exercise and ACJ intake. Thus, the results showed in this clinical study in young triathletes will help to elucidate novel interactions and mechanisms between the excretion of lipid peroxidation metabolites from CNS, supplementation of polyphenols-rich juice in the diet and physical exercise during a training season.

Same Performance Changes after Live High-Train Low in Normobaric vs. Hypobaric Hypoxia.

PURPOSE: We investigated the changes in physiological and performance parameters after a Live High-Train Low (LHTL) altitude camp in normobaric (NH) or hypobaric hypoxia (HH) to reproduce the actual training practices of endurance athletes using a crossover-designed study. METHODS: Well-trained triathletes (n = 16) were split into two groups and completed two 18-day LTHL camps during which they trained at 1100-1200 m and lived at 2250 m (P i O2 = 111.9 ± 0.6 vs. 111.6 ± 0.6 mmHg) under NH (hypoxic chamber; FiO2 18.05 ± 0.03%) or HH (real altitude; barometric pressure 580.2 ± 2.9 mmHg) conditions. The subjects completed the NH and HH camps with a 1-year washout period. Measurements and protocol were identical for both phases of the crossover study. Oxygen saturation (S p O2) was constantly recorded nightly. P i O2 and training loads were matched daily. Blood samples and VO2max were measured before (Pre-) and 1 day after (Post-1) LHTL. A 3-km running-test was performed near sea level before and 1, 7, and 21 days after training camps. RESULTS: Total hypoxic exposure was lower for NH than for HH during LHTL (230 vs. 310 h; P < 0.001). Nocturnal S p O2 was higher in NH than in HH (92.4 ± 1.2 vs. 91.3 ± 1.0%, P < 0.001). VO2max increased to the same extent for NH and HH (4.9 ± 5.6 vs. 3.2 ± 5.1%). No difference was found in hematological parameters. The 3-km run time was significantly faster in both conditions 21 days after LHTL (4.5 ± 5.0 vs. 6.2 ± 6.4% for NH and HH), and no difference between conditions was found at any time. CONCLUSION: Increases in VO2max and performance enhancement were similar between NH and HH conditions.

[Not Available]. / Análisis de la edad morfológica en nadadores y triatletas adolescentes.

El proceso de formación de futuros deportistas debe ser un trabajo estructurado y planificado para poder alcanzar el máximo nivel deportivo. Es fundamental en este periodo tener presentes los ritmos de desarrollo y maduración de los jóvenes deportistas para así adecuar las cargas de entrenamientos a sus estados evolutivos. El objetivo del estudio fue determinar y analizar la edad morfológica en nadadores y triatletas adolescentes, estableciendo diferencias entre su edad cronológica, grupos y género. A través del método antropométrico se determinó el estado de maduración biológica en un grupo de 37 deportistas jóvenes tecnificados. Los resultados muestran que la mayoría de la muestra (70,8%) se encuentra en un estado avanzado de desarrollo con respecto a su edad cronológica, siendo más notorio en el caso de los nadadores este estado de madurez avanzado. Tener una edad morfológica más avanzada respecto a su edad cronológica podría favorecer a los deportistas adolescentes a la hora de conseguir mejores marcas y resultados en las competiciones y de esta manera acceder con más facilidad a los programas de tecnificación. El índice de desarrollo corporal modificado (IDCm) se presenta como un método validado, fiable y no invasivo para tener presente el grado de desarrollo y maduración en la selección de talentos deportivos y adecuar las cargas de entrenamiento al estado evolutivo de los deportistas.

Análisis de la edad morfológica en nadadores y triatletas adolescentes / Analysis of morphological age in young swimmers and triathletes

El proceso de formación de futuros deportistas debe ser un trabajo estructurado y planificado para poder alcanzar el máximo nivel deportivo. Es fundamental en este periodo tener presentes los ritmos de desarrollo y maduración de los jóvenes deportistas para así adecuar las cargas de entrenamientos a sus estados evolutivos. El objetivo del estudio fue determinar y analizar la edad morfológica en nadadores y triatletas adolescentes, estableciendo diferencias entre su edad cronológica, grupos y género. A través del método antropométrico se determinó el estado de maduración biológica en un grupo de 37 deportistas jóvenes tecnificados. Los resultados muestran que la mayoría de la muestra (70,8%) se encuentra en un estado avanzado de desarrollo con respecto a su edad cronológica, siendo más notorio en el caso de los nadadores este estado de madurez avanzado. Tener una edad morfológica más avanzada respecto a su edad cronológica podría favorecer a los deportistas adolescentes a la hora de conseguir mejores marcas y resultados en las competiciones y de esta manera acceder con más facilidad a los programas de tecnificación. El índice de desarrollo corporal modificado (IDCm) se presenta como un método validado, fiable y no invasivo para tener presente el grado de desarrollo y maduración en la selección de talentos deportivos y adecuar las cargas de entrenamiento al estado evolutivo de los deportistas (AU)

The formation of future athletes must be structured and planned in order to achieve the highest performance level. It is necessary to take into account the rates of development and maturation of young athletes in this early period in order to adjust the training loads to their developmental stages. The aim of this study has been to analyze the morphological age in young swimmers and triathletes to establish differences between their chronological age, groups and gender. Applying the anthropometric method and equations of Siret et al., we determined the biological maturation in 37 young elite athletes. The results showed that most athletes are in an advanced state of development (70.8%) compared to their chronological age. In swimmers this rate of development was more noticeable than in triathletes. If athletes have an advanced morphological age compared to chronological age, these young athletes could obtain better results in competition and enter elite programs more easily. The modified morphological age index (IDCm, from Spanish) is presented like a reliable and non-invasive method to establish the maturation state when we select potential sport talent or when we adapt training loads to the developmental state of athletes (AU)

Assessment of oxidative stress biomarkers - neuroprostanes and dihomo-isoprostanes - in the urine of elite triathletes after two weeks of moderate-altitude training.

This randomized and controlled trial investigated whether the increase in elite training at different altitudes altered the oxidative stress biomarkers of the nervous system. This is the first study to investigate four F4-neuroprostanes (F4-NeuroPs) and four F2-dihomo-isoprostanes (F2-dihomo-IsoPs) quantified in 24-h urine. The quantification was carried out by ultra high pressure liquid chromatography-triple quadrupole-tandem mass spectrometry (UHPLC-QqQ-MS/MS). Sixteen elite triathletes agreed to participate in the project. They were randomized in two groups, a group submitted to altitude training (AT, n = 8) and a group submitted to sea level training (SLT) (n = 8), with a control group (Cg) of non-athletes (n = 8). After the experimental period, the AT group triathletes gave significant data: 17-epi-17-F2t-dihomo-IsoP (from 5.2 ± 1.4 µg/mL 24 h(-1) to 6.6 ± 0.6 µg/mL 24 h(-1)), ent-7(RS)-7-F2t-dihomo-IsoP (from 6.6 ± 1.7 µg/mL 24 h(-1) to 8.6 ± 0.9 µg/mL 24 h(-1)), and ent-7-epi-7-F2t-dihomo-IsoP (from 8.4 ± 2.2 µg/mL 24 h(-1) to 11.3 ± 1.8 µg/mL 24 h(-1)) increased, while, of the neuronal degeneration-related compounds, only 10-epi-10-F4t-NeuroP (8.4 ± 1.7 µg/mL 24 h(-1)) and 10-F4t-NeuroP (5.2 ± 2.9 µg/mL 24 h(-1)) were detected in this group. For the Cg and SLT groups, no significant changes had occurred at the end of the two-week experimental period. Therefore, and as the main conclusion, the training at moderate altitude increased the F4-NeuroPs- and F2-dihomo-isoPs-related oxidative damage of the central nervous system compared to similar training at sea level.

Prooxidant/Antioxidant Balance in Hypoxia: A Cross-Over Study on Normobaric vs. Hypobaric "Live High-Train Low".

"Live High-Train Low" (LHTL) training can alter oxidative status of athletes. This study compared prooxidant/antioxidant balance responses following two LHTL protocols of the same duration and at the same living altitude of 2250 m in either normobaric (NH) or hypobaric (HH) hypoxia. Twenty-four well-trained triathletes underwent the following two 18-day LHTL protocols in a cross-over and randomized manner: Living altitude (PIO2 = 111.9 ± 0.6 vs. 111.6 ± 0.6 mmHg in NH and HH, respectively); training "natural" altitude (~1000-1100 m) and training loads were precisely matched between both LHTL protocols. Plasma levels of oxidative stress [advanced oxidation protein products (AOPP) and nitrotyrosine] and antioxidant markers [ferric-reducing antioxidant power (FRAP), superoxide dismutase (SOD) and catalase], NO metabolism end-products (NOx) and uric acid (UA) were determined before (Pre) and after (Post) the LHTL. Cumulative hypoxic exposure was lower during the NH (229 ± 6 hrs.) compared to the HH (310 ± 4 hrs.; P<0.01) protocol. Following the LHTL, the concentration of AOPP decreased (-27%; P<0.01) and nitrotyrosine increased (+67%; P<0.05) in HH only. FRAP was decreased (-27%; P<0.05) after the NH while was SOD and UA were only increased following the HH (SOD: +54%; P<0.01 and UA: +15%; P<0.01). Catalase activity was increased in the NH only (+20%; P<0.05). These data suggest that 18-days of LHTL performed in either NH or HH differentially affect oxidative status of athletes. Higher oxidative stress levels following the HH LHTL might be explained by the higher overall hypoxic dose and different physiological responses between the NH and HH.